Description
Human NCK1 Recombinant Protein (N-His)
Human NCK1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala2-Ser377, with an N-terminal His tag. Predicted molecular weight: 45.03 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. SH2/SH3 adapter protein NCK1 is a ~42 kDa protein. Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. For research use only.
Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA.
| Product Name: | Human NCK1 Recombinant Protein (N-His) |
| SKU: | GXRP01039 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | SH2/SH3 adaptor protein NCK-alpha, Nck-1, NCK adaptor protein 1, NCK, NCK1, Cytoplasmic protein NCK1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala2-Ser377 |
| Calculated MW: | 45.03 kDa |
| UniProt: | P16333 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
