The Human NE ELA2 (Elastase-2 Neutrophil) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of NE ELA2 levels in human samples such as serum, plasma, and cell culture supernatants. NE ELA2, also known as neutrophil elastase, is a key enzyme produced by neutrophils and is involved in various inflammatory processes and immune responses. Elevated levels of NE ELA2 have been associated with conditions such as chronic obstructive pulmonary disease (COPD), acute respiratory distress syndrome (ARDS), and cystic fibrosis, making it an important biomarker for studying and monitoring these diseases.
This CLIA kit offers a high level of specificity and reproducibility, ensuring reliable results for researchers studying the role of NE ELA2 in inflammation, infection, and respiratory disorders. Its ease of use and versatility make it suitable for a wide range of research applications in the field of immunology and respiratory medicine.
Product Name:
Human NE/ELA2 (Elastase 2, Neutrophil) CLIA Kit
SKU:
HUES01066
Target:
Human NE/ELA2 (Elastase 2, Neutrophil)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NE/ELA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NE/ELA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NE/ELA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NE/ELA2. You can calculate the concentration of Human NE/ELA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
98-115
90-104
Average (%)
92
105
97
1:4
Range (%)
93-106
84-99
89-101
Average (%)
99
91
94
1:8
Range (%)
102-116
101-117
99-117
Average (%)
108
109
107
1:16
Range (%)
98-112
101-114
88-99
Average (%)
104
107
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
103
EDTA plasma (n=5)
97-111
102
Cell culture media (n=5)
98-112
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.15
4.33
0.49
1.21
4.41
Standard deviation
0.04
0.09
0.27
0.05
0.09
0.41
C V (%)
8.33
7.83
6.24
10.2
7.44
9.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NE/ELA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NE/ELA2 in samples. No significant cross-reactivity or interference between Human NE/ELA2 and analogues was observed.
