The Human NE (Elastase-2 Neutrophil) ELISA Kit is specifically developed for the precise quantification of elastase-2 neutrophil levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.Elastase-2 neutrophil, also known as NE, is a key enzyme involved in immune response regulation and inflammatory processes. Abnormal levels of NE have been linked to various diseases such as chronic obstructive pulmonary disease (COPD), cystic fibrosis, and acute respiratory distress syndrome (ARDS), highlighting its importance as a biomarker in clinical research. With the Human NE ELISA Kit, researchers can reliably measure NE levels in human samples, facilitating the study of neutrophil function and its implications in disease pathogenesis. This kit is a valuable tool for investigating potential therapeutic targets and developing innovative treatment strategies for NE-related disorders.
Product Name:
Human NE/ELA2 (Elastase 2, Neutrophil) ELISA Kit
SKU:
HUES02889
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
