Human NF-kBp65 (Nuclear Factor Kappa B p65) CLIA Kit
The Human NF-kB p65 (Nuclear Factor Kappa-B p65) CLIA Kit is specifically designed for the quantitative measurement of NF-kB p65 levels in human cell lysates and tissue homogenates. This kit offers high sensitivity and precision, ensuring accurate and reliable results for research applications.NF-kB p65 is a key transcription factor involved in the regulation of immune and inflammatory responses, as well as cell growth and survival. Dysregulation of NF-kB p65 has been implicated in various diseases such as cancer, inflammatory disorders, and autoimmune diseases.
Therefore, accurate measurement of NF-kB p65 levels is crucial for understanding its role in these pathologies and developing potential therapeutic strategies.The Human NF-kB p65 CLIA Kit provides a valuable tool for researchers studying NF-kB p65 signaling pathways and exploring its implications in disease mechanisms. With its high-quality components and user-friendly protocol, this kit is an essential resource for advancing research in the field of molecular biology and immunology.
Product Name:
Human NF-kBp65 (Nuclear Factor Kappa B p65) CLIA Kit
SKU:
HUES00797
Target:
Human NF-kBp65 (Nuclear Factor Kappa B p65)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NF-κB p65. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NF-κB p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NF-κB p65, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NF-κB p65. You can calculate the concentration of Human NF-κB p65 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
92-104
87-99
Average (%)
100
98
92
1:4
Range (%)
101-115
96-111
100-113
Average (%)
107
103
107
1:8
Range (%)
101-118
96-108
98-116
Average (%)
108
102
106
1:16
Range (%)
88-98
85-98
95-109
Average (%)
93
90
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
104
EDTA plasma (n=5)
99-113
105
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.99
203.7
965.39
100.87
215.26
983.84
Standard deviation
11.57
23.34
81.09
11.84
20.56
96.22
C V (%)
10.71
11.46
8.4
11.74
9.55
9.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NF-κB p65 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NF-κB p65 in samples. No significant cross-reactivity or interference between Human NF-κB p65 and analogues was observed.
