The Human NFKB Repressing Factor (NKRF) ELISA Kit is a powerful tool for accurately quantifying levels of NKRF in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering precise and consistent results for a variety of research purposes.NKRF is a key protein involved in regulating the NFKB signaling pathway, playing a critical role in inflammatory responses, immune regulation, and cell survival. Dysregulation of NFKB signaling has been implicated in a range of diseases, including cancer, autoimmune disorders, and inflammatory conditions.
The ability to measure NKRF levels can provide valuable insights into these conditions, aiding in the development of targeted therapies and diagnostic approaches.Don't miss out on the opportunity to explore the intricate mechanisms of NFKB signaling with the Human NFKB Repressing Factor (NKRF) ELISA Kit. Unlock new possibilities for your research and gain a deeper understanding of the complexities of disease pathways.
Product Name:
Human NFKB Repressing Factor (NKRF) ELISA Kit
Product Code:
HUDL02045
Size:
96 Assays
Target:
NKRF
Synonyms:
ITBA4, NRF, NF-Kappa B Repressing Factor, Transcription factor NRF
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
