The Human NFkB (Nuclear Factor Kappa B) ELISA Kit is specifically designed for the precise measurement of NFkB levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and accuracy, ensuring consistent and reliable results for a variety of research purposes.NFkB is a key transcription factor that plays a critical role in regulating the immune response, inflammation, and cell survival. Dysregulation of NFkB has been linked to various diseases including cancer, inflammatory disorders, and autoimmune conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions. With the Human NFkB ELISA Kit, researchers can confidently assess NFkB levels in human samples, advancing their understanding of NFkB signaling and its implications in disease pathogenesis. Trust this kit to deliver robust and reproducible data for your research needs.
Product Name:
Human NFkB (Nuclear Factor Kappa B) ELISA Kit
SKU:
HUES02429
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
