The Human NGB (Neuroglobin) CLIA Kit is specifically designed to accurately measure neuroglobin levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential tool for various research applications.Neuroglobin is a key protein involved in neuroprotection and oxygen homeostasis in the brain.
It plays a crucial role in conditions like neurodegenerative disorders, stroke, and brain injury, making it an important biomarker for studying these conditions and developing potential treatments.Get precise and reliable measurements of neuroglobin levels with the Human NGB (Neuroglobin) CLIA Kit from Assaygenie. Order yours today and advance your research in neuroscience and neurodegenerative diseases.
Product Name:
Human NGB (Neuroglobin) CLIA Kit
SKU:
HUES00987
Target:
Human NGB (Neuroglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NGB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NGB. You can calculate the concentration of Human NGB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
89-103
86-99
Average (%)
100
95
91
1:4
Range (%)
97-111
101-113
91-104
Average (%)
103
106
96
1:8
Range (%)
88-104
88-101
101-118
Average (%)
95
95
107
1:16
Range (%)
93-107
89-103
90-106
Average (%)
100
96
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
104
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.37
198.49
718.54
91.37
208.87
754.45
Standard deviation
9.98
14.95
58.7
11.64
16.98
50.32
C V (%)
10.04
7.53
8.17
12.74
8.13
6.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NGB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NGB in samples. No significant cross-reactivity or interference between Human NGB and analogues was observed.
