Description
Human NISCH Recombinant Protein (N-His)
Human NISCH Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp1231-Glu1501, with an N-terminal His tag. Predicted molecular weight: 32.99 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Nischarin is a ~166 kDa protein. Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. For research use only.
Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways.
| Product Name: | Human NISCH Recombinant Protein (N-His) |
| SKU: | GXRP12331 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | I-1, I-1 receptor candidate protein, I1R, I1R candidate protein, IR1, IRAS, Imidazoline receptor 1, Imidazoline receptor antisera-selected protein, Imidazoline-1 receptor, Imidazoline-1 receptor candidate protein, KIAA0975, NISCH, Nischarin, hIRAS |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asp1231-Glu1501 |
| Calculated MW: | 32.99 kDa |
| UniProt: | Q9Y2I1 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
