Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit
The Human NOS2 (iNOS, Nitric Oxide Synthase 2, Inducible) CLIA Kit is specifically designed for the accurate detection of NOS2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.NOS2 is an enzyme that plays a crucial role in the production of nitric oxide, a key signaling molecule involved in various physiological processes.
Dysregulation of NOS2 has been linked to inflammatory conditions, cardiovascular diseases, and neurodegenerative disorders, making it an important biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Human NOS2 CLIA Kit offers researchers a valuable tool for studying the role of NOS2 in health and disease, providing accurate and precise measurements for a wide range of samples.
Product Name:
Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit
SKU:
HUES00472
Target:
Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NOS2/iNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NOS2/iNOS. You can calculate the concentration of Human NOS2/iNOS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
88-101
101-115
Average (%)
95
94
107
1:4
Range (%)
89-105
99-114
90-104
Average (%)
96
105
97
1:8
Range (%)
103-118
96-109
92-107
Average (%)
108
103
98
1:16
Range (%)
101-116
98-114
98-113
Average (%)
106
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
95
EDTA plasma (n=5)
88-103
95
Cell culture media (n=5)
92-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.47
190.32
883.12
105.04
190.48
951.04
Standard deviation
8.8
14.39
55.28
8.45
16.04
65.81
C V (%)
9.12
7.56
6.26
8.04
8.42
6.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NOS2/iNOS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed.
