Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
The Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is a cutting-edge tool designed to quantitatively measure the levels of Nitric Oxide Synthase 2, also known as Inducible Nitric Oxide Synthase (iNOS), in various biological samples. iNOS is a significant enzyme that plays a crucial role in the production of nitric oxide, a key signaling molecule involved in a wide range of physiological and pathophysiological processes. Accurate measurement of iNOS levels is essential for understanding its involvement in immune responses, inflammation, and diseases where nitric oxide signaling is implicated. The Rat NOS2/iNOS ELISA Kit by Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this ELISA kit provides reliable performance and user-friendly protocols, making it an excellent choice for research focusing on nitric oxide signaling pathways and related mechanisms.
Product Name:
Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
SKU:
AEES00441
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
