Description
Human NOS2 Recombinant Protein (N-His)
Human NOS2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met533-Lys696, with an N-terminal His tag. Predicted molecular weight: 20.42 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Nitric oxide synthase, inducible (NOS2/iNOS) is a ~131 kDa protein. Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. For research use only.
In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.
| Product Name: | Human NOS2 Recombinant Protein (N-His) |
| SKU: | GXRP01473 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Nitric oxide synthase, inducible, NOS2A, Inducible NO synthase, HEP-NOS, Hepatocyte NOS, Peptidyl-cysteine S-nitrosylase NOS2, iNOS, NOS2, Inducible NOS, NOS type II |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met533-Lys696 |
| Calculated MW: | 20.42 kDa |
| UniProt: | P35228 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
