Description
Human NPC1 Recombinant Protein (N-His)
Human NPC1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Arg372-Phe622, with an N-terminal His tag. Predicted molecular weight: 31.01 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. NPC intracellular cholesterol transporter 1 (NPC1) is a ~142 kDa protein. Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. For research use only.
Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable).
| Product Name: | Human NPC1 Recombinant Protein (N-His) |
| SKU: | GXRP05752 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | NPC intracellular cholesterol transporter 1, Niemann-Pick C1 protein, NPC1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Arg372-Phe622 |
| Calculated MW: | 31.01 kDa |
| UniProt: | O15118 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
