The Human NTN1 (Netrin-1) ELISA Kit is specifically designed for the precise measurement of Netrin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Netrin-1 is a key protein involved in axon guidance and cell migration, playing a critical role in nervous system development and regeneration. Dysregulation of Netrin-1 has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment options.Overall, the Human NTN1 ELISA Kit provides researchers with a reliable tool to investigate the role of Netrin-1 in different pathologies and gain a deeper understanding of its therapeutic implications.
Product Name:
Human Ntn1 (Netrin 1) ELISA Kit
SKU:
HUES03203
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
