Human Paraneoplastic Antigen MA2 (PNMA2) ELISA Kit
The Human Paraneoplastic Antigen MA2 (PNMA2) ELISA Kit is a cutting-edge tool designed for the precise measurement of PNMA2 levels in human serum, plasma, and cell culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, ensuring accurate and dependable results for a diverse range of research applications.PNMA2, also known as Ma2, is a critical antigen associated with paraneoplastic neurological syndromes, including limbic encephalitis and other autoimmune disorders. By detecting PNMA2 levels, researchers can gain valuable insights into the pathogenesis of these conditions, facilitating the development of novel diagnostic and therapeutic strategies.
With its user-friendly protocol and high-performance components, the Human Paraneoplastic Antigen MA2 (PNMA2) ELISA Kit is an indispensable resource for investigating the role of PNMA2 in disease progression and exploring its potential as a diagnostic marker. Trust in this innovative kit to elevate your research and advance our understanding of paraneoplastic antigen biology.
Product Name:
Human Paraneoplastic Antigen MA2 (PNMA2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
