The Human PDCN (Podocin) ELISA Kit is specifically designed for the precise measurement of podocin levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Podocin is a critical protein that plays a key role in maintaining the integrity of the kidney filtration barrier. Dysregulation of podocin is associated with various kidney diseases, including nephrotic syndrome and diabetic nephropathy. Therefore, the Human PDCN ELISA Kit serves as a valuable tool for studying these conditions and developing potential therapeutic interventions.Overall, the Human PDCN ELISA Kit from Assay Genie provides researchers with a reliable and efficient method for studying podocin levels in biological samples, offering valuable insights into kidney function and related diseases.
Product Name:
Human PDCN (Podocin) ELISA Kit
SKU:
HUES02037
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
