The Human Phenylalanine Hydroxylase (PAH) ELISA Kit is specifically designed for detecting levels of phenylalanine hydroxylase in human serum, plasma, and cell culture supernatants with high sensitivity and specificity. This kit provides accurate and reproducible results, making it a valuable tool for various research applications.Phenylalanine hydroxylase is an essential enzyme involved in the breakdown of phenylalanine, an amino acid found in many proteins. Mutations in the PAH gene can lead to phenylketonuria (PKU), a metabolic disorder that can result in intellectual disabilities and other health complications if not treated early.
Monitoring phenylalanine hydroxylase levels is crucial for managing PKU and developing new treatment strategies.With its reliability and precision, the Human Phenylalanine Hydroxylase ELISA Kit is an indispensable resource for scientists studying PKU, metabolic disorders, and related conditions. Get accurate and insightful results with this advanced ELISA kit from Assay Genie.
Product Name:
Human Phenylalanine Hydroxylase (PAH) ELISA Kit
Product Code:
HUDL02172
Size:
96 Assays
Target:
PAH
Synonyms:
PKU, PKU1, Phe-4-monooxygenase
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
