Description
Human PHF2 Recombinant Protein (N-His)
Human PHF2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln83-Arg450, with an N-terminal His tag. Predicted molecular weight: 44.27 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Lysine-specific demethylase PHF2 is a ~120 kDa protein. Lysine demethylase that demethylates both histones and non-histone proteins. For research use only.
Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
| Product Name: | Human PHF2 Recombinant Protein (N-His) |
| SKU: | GXRP09725 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Lysine-specific demethylase PHF2; 1.14.11.-; GRC5; PHD finger protein 2; PHF2; CENP-35; KIAA0662 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln83-Arg450 |
| Calculated MW: | 44.27 kDa |
| UniProt: | O75151 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
