Description
Human PIK3CA Recombinant Protein (N-His)
Human PIK3CA Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn345-Phe550, with an N-terminal His tag. Predicted molecular weight: 25.73 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA) is a ~124 kDa protein. Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. For research use only.
Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF.
| Product Name: | Human PIK3CA Recombinant Protein (N-His) |
| SKU: | GXRP03424 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Phosphoinositide-3-kinase catalytic alpha polypeptide, PIK3CA, PtdIns-3-kinase subunit p110-alpha, PI3-kinase subunit alpha, p110alpha, Serine/threonine protein kinase PIK3CA, PI3K-alpha, Phosphoinositide 3-kinase alpha, Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn345-Phe550 |
| Calculated MW: | 25.73 kDa |
| UniProt: | P42336 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
