Description
Human PLA2G2A Recombinant Protein (N-His)
Human PLA2G2A Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn21-Cys144, with an N-terminal His tag. Predicted molecular weight: 16.21 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Phospholipase A2, membrane associated (PLA2G2A) is a ~16 kDa protein. Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. For research use only.
Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes.
| Product Name: | Human PLA2G2A Recombinant Protein (N-His) |
| SKU: | GXRP00974 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Phosphatidylcholine 2-acylhydrolase 2A, NPS-PLA2, RASF-A, Non-pancreatic secretory phospholipase A2, Group IIA phospholipase A2, GIIC sPLA2, PLA2L, PLA2G2A, PLA2B, Phospholipase A2, membrane associated |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn21-Cys144 |
| Calculated MW: | 16.21 kDa |
| UniProt: | P14555 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
