Human Polymerase DNA Directed Gamma 1 (POLg1) ELISA Kit
The Human Polymerase DNA-Directed Gamma 1 (POLG1) ELISA Kit is a cutting-edge tool for the precise measurement of POLG1 levels in human samples including serum, plasma, and cell culture supernatants. This kit is equipped with superior sensitivity and specificity, guaranteeing trustworthy and consistent results for various research studies.POLG1 is a key enzyme involved in DNA replication and repair, critical for maintaining genomic stability. Dysregulation of POLG1 has been linked to a variety of diseases such as mitochondrial disorders, neurodegenerative conditions, and certain types of cancer.
Accurate measurement of POLG1 levels can provide valuable insights into disease mechanisms and aid in the development of potential therapeutic interventions.With its advanced technology and reliable performance, the Human POLG1 ELISA Kit is an essential tool for researchers dedicated to unraveling the complexities of DNA metabolism and its implications in human health and disease.
Product Name:
Human Polymerase DNA Directed Gamma 1 (POLg1) ELISA Kit
Product Code:
HUDL02362
Size:
96 Assays
Target:
POLg1
Synonyms:
PEO, POLG1, POLGA, SANDO, SCAE, Mitochondrial DNA polymerase catalytic subunit
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-103
94
Heparin plasma (n=5)
87-99
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
