The Human PPP3R1 (Calcineurin B) ELISA Kit is specifically designed for the accurate and precise measurement of PPP3R1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results that are essential for a variety of research applications. PPP3R1, also known as Calcineurin B, is a key regulatory subunit of the calcineurin protein phosphatase complex. This complex plays a crucial role in various cellular processes, including signal transduction, immune response, and gene expression.
Dysregulation of PPP3R1 has been linked to several diseases, such as autoimmune disorders, cardiac hypertrophy, and neurodegenerative conditions. By accurately measuring PPP3R1 levels, researchers can gain valuable insights into its role in disease pathogenesis and develop potential therapeutic strategies targeting the calcineurin pathway. This ELISA kit provides a valuable tool for studying PPP3R1 and its implications in various health conditions.
Product Name:
Human PPP3R1/Calcineurin B ELISA Kit
SKU:
HUFI01212
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-104%
88-101%
87-96%
EDTA Plasma (n = 5)
86-104%
83-99%
81-97%
Heparin Plasma (n = 5)
85-97%
87-101%
87-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-100
95
EDTA Plasma (n = 5)
85-104
96
Heparin Plasma (n = 5)
86-98
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
