The Human PRF1 (Perforin-1) ELISA Kit is specifically designed for the quantitative detection of perforin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Perforin-1 is a key protein involved in the immune response, particularly in cytotoxic T lymphocytes and natural killer cells. It plays a crucial role in the elimination of target cells, making it an important biomarker for studying immune function and disorders such as autoimmune diseases, infections, and cancer.With its advanced technology and user-friendly format, the Human PRF1 ELISA Kit is a valuable tool for researchers looking to explore the role of perforin-1 in health and disease, leading to potential new insights and therapeutic strategies.
Product Name:
Human PRF1 (Perforin 1) ELISA Kit
SKU:
HUES02213
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
