Description
Human PRKAA2 Recombinant Protein (N-His)
Human PRKAA2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Arg552, with an N-terminal His tag. Predicted molecular weight: 64.48 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. 5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) is a ~62 kDa protein. Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. For research use only.
In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2).
| Product Name: | Human PRKAA2 Recombinant Protein (N-His) |
| SKU: | GXRP01804 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | PRKAA2, Acetyl-CoA carboxylase kinase, AMPK subunit alpha-2, ACACA kinase, AMPK, AMPK2, Hydroxymethylglutaryl-CoA reductase kinase, 5'-AMP-activated protein kinase catalytic subunit alpha-2, HMGCR kinase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Arg552 |
| Calculated MW: | 64.48 kDa |
| UniProt: | P54646 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
