Description
Human PRKACA Recombinant Protein (N-His)
Human PRKACA Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning His40-Lys310, with an N-terminal His tag. Predicted molecular weight: 33.95 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. cAMP-dependent protein kinase catalytic subunit alpha (PRKACA) is a ~40 kDa protein. Phosphorylates a large number of substrates in the cytoplasm and the nucleus. For research use only.
Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, histone H1.4 (H1-4), PSMC5/RPT6, PJA2, RYR2, RORA, SOX9, UHRF1 and VASP. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.
| Product Name: | Human PRKACA Recombinant Protein (N-His) |
| SKU: | GXRP04391 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | PRKACA, PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | His40-Lys310 |
| Calculated MW: | 33.95 kDa |
| UniProt: | P17612 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
