Human Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 2 (PLOD2) ELISA Kit
The Human Procollagen Lysine 2-Oxoglutarate 5-Dioxygenase-2 (PLod2) ELISA Kit is specifically designed for the precise measurement of PLod2 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and consistent results, making it an invaluable tool for various research applications.PLod2, also known as lysyl hydroxylase 2, is a key enzyme involved in collagen biosynthesis, playing a critical role in maintaining the structural integrity of connective tissues.
Dysregulation of PLod2 has been implicated in diseases such as fibrosis, cancer, and cardiovascular disorders, highlighting its significance as a potential biomarker for disease progression and therapeutic target identification.This ELISA kit provides researchers with a comprehensive solution for studying the role of PLod2 in disease pathogenesis and developing novel treatment strategies. Trust in the accuracy and precision of the Human Procollagen Lysine 2-Oxoglutarate 5-Dioxygenase-2 (PLod2) ELISA Kit for your research needs.
Product Name:
Human Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 2 (PLOD2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
