Human Programmed cell death protein 2 (PDCD2) ELISA Kit
The Human PDCD2 (Programmed Cell Death Protein 2) ELISA Kit is specifically designed for the precise measurement of PDCD2 levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and accuracy, guaranteeing dependable and consistent results that are suitable for a diverse range of research purposes.PDCD2 is a key protein involved in programmed cell death, influencing cell survival and growth regulation. Its role in various diseases such as cancer, autoimmune disorders, and viral infections makes it a valuable biomarker for investigating disease mechanisms and exploring therapeutic interventions.
Utilizing the Human PDCD2 ELISA Kit allows researchers to accurately assess PDCD2 levels in biological samples, providing valuable insights into cellular pathways and potential therapeutic targets. With its reliability and precision, this kit is an indispensable tool for advancing research in cell death biology and related fields.
Product Name:
Human Programmed cell death protein 2 (PDCD2) ELISA Kit
Product Code:
HUDL02221
Size:
96 Assays
Target:
PDCD2
Synonyms:
RP8, ZMYND7, Zinc finger MYND domain-containing protein 7, Zinc finger protein Rp-8
Detection Method:
Sandwich
Range:
78.1-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
