Human Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) ELISA Kit
The Human Protein Phosphatase 2A Activator Regulatory Subunit 4 (PPP2R4) ELISA Kit is a comprehensive kit designed for the accurate measurement of PPP2R4 protein levels in human samples such as serum, plasma, and cell culture supernatants. This kit is characterized by its high sensitivity and specificity, ensuring precise and reproducible results that are suitable for a variety of research applications.PPP2R4 is a critical regulatory subunit of protein phosphatase 2A, playing a key role in the regulation of cell growth, cell cycle progression, and signal transduction. Dysregulation of PPP2R4 has been implicated in various diseases, including cancer, neurological disorders, and autoimmune conditions.
As such, accurate detection of PPP2R4 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.The Human PPP2R4 ELISA Kit from Assay Genie offers researchers a reliable tool to study the function and dysregulation of PPP2R4 in various disease contexts, aiding in the development of novel treatment strategies and biomarkers. With its high precision and ease of use, this kit is a valuable resource for advancing scientific understanding in the field of protein phosphatase research.
Product Name:
Human Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
