Rat Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) ELISA Kit
The Rat Protein Phosphatase 2A Activator Regulatory Subunit 4 (PPP2R4) ELISA Kit is specifically designed for the precise measurement of PPP2R4 levels in rat samples such as serum, plasma, and cell lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.PPP2R4 is a critical regulator of protein phosphatase 2A, an enzyme involved in numerous cellular processes including cell growth, apoptosis, and signal transduction.
Dysregulation of PPP2R4 has been associated with various diseases, making it a valuable biomarker for studying these conditions and potentially developing new therapeutic interventions.With its high-performance capabilities, the Rat PPP2R4 ELISA Kit provides researchers with a powerful tool for investigating the role of PPP2R4 in biological pathways and disease mechanisms. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Rat Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
