Human Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) ELISA Kit
The Human Protein Phosphatase Mg2+/Mn2+ Dependent 1A (PPM1A) ELISA Kit is a reliable and accurate tool for measuring levels of PPM1A in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for precise and reproducible results for a variety of research applications.PPM1A is an important enzyme involved in cellular signaling pathways, specifically in the regulation of protein phosphorylation. Dysregulation of PPM1A has been associated with various diseases, including cancer, neurodegenerative disorders, and metabolic disorders.
As such, this ELISA kit is valuable for studying the role of PPM1A in these conditions and exploring potential therapeutic interventions.Overall, the Human Protein Phosphatase Mg2+/Mn2+ Dependent 1A (PPM1A) ELISA Kit is a valuable tool for researchers seeking to investigate the physiological and pathological functions of PPM1A and its potential implications in various disease states.
Product Name:
Human Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) ELISA Kit
Product Code:
HUDL02378
Size:
96 Assays
Target:
PPM1A
Synonyms:
PP2C-ALPHA, PP2CA, Phosphatase 2C Alpha, Protein Phosphatase 1A(Formerly 2C)Magnesium-Dependent,Alpha
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
