Rat Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) ELISA Kit
The Rat Protein Phosphatase Mg2/Mn2-Dependent 1A (PPM1A) ELISA Kit is a powerful tool for accurately measuring levels of PPM1A in rat samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures reliable and reproducible results for a variety of research applications.PPM1A is a key enzyme involved in regulating cellular processes by dephosphorylating proteins, thereby playing a critical role in cell signaling pathways. Dysregulation of PPM1A activity has been linked to various diseases including cancer, metabolic disorders, and neurological conditions, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.
Overall, the Rat Protein Phosphatase Mg2/Mn2-Dependent 1A (PPM1A) ELISA Kit offers researchers a precise and efficient way to quantify PPM1A levels in rat samples, facilitating in-depth studies of its biological functions and implications in disease pathogenesis.
Product Name:
Rat Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) ELISA Kit
Product Code:
RTDL00858
Size:
96 Assays
Target:
PPM1A
Synonyms:
PP2C-ALPHA, PP2CA, Phosphatase 2C Alpha, Protein Phosphatase 1A(Formerly 2C)Magnesium-Dependent,Alpha
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
