SynonymsM2PK,PKM,M2-PK,PKM1,CTHBP,OIP3,PK3,PKM,TCB,THBP1,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,Cytosolic thyroid hormone-binding protein
ReactivityHuman
Sample TypeSerum, Plasma, Tissue Homogenates And Other Biological Fluids
SKU:
HUDL02309
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P14618
Sensitivity:
29pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
M2PK,PKM,M2-PK,PKM1,CTHBP,OIP3,PK3,PKM,TCB,THBP1,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,Cytosolic thyroid hormone-binding protein
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
The Human Pyruvate Kinase Muscle (PKM2) ELISA Kit is specially designed for the precise measurement of PKM2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results that are perfect for a variety of research applications.PKM2 is a key enzyme in glycolysis and has been linked to various diseases including cancer, metabolic disorders, and cardiovascular diseases.
Understanding PKM2 levels is crucial for studying the underlying mechanisms of these diseases and developing targeted therapies.With the Human Pyruvate Kinase Muscle (PKM2) ELISA Kit, researchers can confidently analyze PKM2 levels in human samples, leading to valuable insights and advancements in the field of medical research.
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
