The Human PKM2 (Pyruvate Kinase, Muscle) ELISA Kit is a specialized assay designed for the precise quantitative analysis of Pyruvate Kinase M2, also known as PKM2, in various biological samples. PKM2 is a critical enzyme involved in glycolysis regulation and metabolic processes essential for cell function and energy production. Accurate measurement of PKM2 levels is crucial for understanding its role in metabolic pathways, cell proliferation, and differentiation. This ELISA kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control standards, the PKM2 ELISA Kit delivers robust performance while maintaining ease of use, making it an excellent choice for both research and clinical applications. Researchers can trust the accuracy and dependability of Assay Genie's PKM2 ELISA Kit for quantifying this vital biomarker in their studies.
Product Name:
Human PKM2 (Pyruvate Kinase, Muscle) ELISA Kit
SKU:
AEES00099
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 IU/mL
Detection range:
15.63-1000 IU/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
