Description
Human RLIM Recombinant Protein (N-His)
Human RLIM Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser97-Ser370, with an N-terminal His tag. Predicted molecular weight: 32.94 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E3 ubiquitin-protein ligase RLIM is a ~68 kDa protein. E3 ubiquitin-protein ligase that acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. For research use only.
Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42/REX1 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals. E3 ubiquitin-protein ligase is required for proper regulation of neural cell differentiation from embryonic stem cells.
| Product Name: | Human RLIM Recombinant Protein (N-His) |
| SKU: | GXRP04678 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | RING-type E3 ubiquitin transferase RLIM, RING finger LIM domain-binding protein, RNF12, R-LIM, Renal carcinoma antigen NY-REN-43, RING finger protein 12, E3 ubiquitin-protein ligase RLIM, RLIM, LIM domain-interacting RING finger protein |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser97-Ser370 |
| Calculated MW: | 32.94 kDa |
| UniProt: | Q9NVW2 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
