Description
Human RSPO2 Recombinant Protein (N-His)
Human RSPO2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser36-Gln243, with an N-terminal His tag. Predicted molecular weight: 26.33 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. R-spondin-2 is a ~28 kDa protein. Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. For research use only.
Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form. 1. de Lau, W. et al. (2011) Nature 476, 293-7. PMID: 21727895 2. Glinka, A. et al. (2011) EMBO reports 12, 1055-61. PMID: 21909076 3. Gong, X. et al. (2012) PloS one 7, e37137. PMID: 22615920 4. Szenker-Ravi, E. et al. (2018) Nature 557, 564-569. PMID: 29769720
| Product Name: | Human RSPO2 Recombinant Protein (N-His) |
| SKU: | GXRP10477 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | R-spondin-2, RSPO2, Roof plate-specific spondin-2, hRspo2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser36-Gln243 |
| Calculated MW: | 26.33 kDa |
| UniProt: | Q6UXX9 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
