Description
Human RTCA Recombinant Protein (N-His)
Human RTCA Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Leu366, with an N-terminal His tag. Predicted molecular weight: 41.50 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. RNA 3'-terminal phosphate cyclase (RTCA) is a ~39 kDa protein. Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. For research use only.
The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. Likely functions in some aspects of cellular RNA processing. Function plays an important role in regulating axon regeneration by inhibiting central nervous system (CNS) axon regeneration following optic nerve injury.
| Product Name: | Human RTCA Recombinant Protein (N-His) |
| SKU: | GXRP07290 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | RNA 3'-terminal phosphate cyclase, RNA cyclase, RNA-3'-phosphate cyclase, 6.5.1.4, RNA terminal phosphate cyclase domain-containing protein 1, RTC domain-containing protein 1, RTCA, RPC, RPC1, RTC1, RTCD1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Leu366 |
| Calculated MW: | 41.50 kDa |
| UniProt: | O00442 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
