Description
Human SCAF4 Recombinant Protein (N-His)
Human SCAF4 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Glu501-Lys635, with an N-terminal His tag. Predicted molecular weight: 17.66 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. SR-related and CTD-associated factor 4 is a ~125 kDa protein. Anti-terminator protein required to prevent early mRNA termination during transcription. For research use only.
Together with SCAF8, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF8, also acts as a suppressor of transcriptional readthrough.
| Product Name: | Human SCAF4 Recombinant Protein (N-His) |
| SKU: | GXRP11248 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CTD-binding SR-like protein RA4, KIAA1172, SCAF4, SFRS15, SR-related and CTD-associated factor 4, Splicing factor, arginine/serine-rich 15 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Glu501-Lys635 |
| Calculated MW: | 17.66 kDa |
| UniProt: | O95104 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
