Description
Human SCAP Recombinant Protein (N-His)
Human SCAP Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser1063-Asp1279, with an N-terminal His tag. Predicted molecular weight: 25.40 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Sterol regulatory element-binding protein cleavage-activating protein is a ~139 kDa protein. Escort protein required for cholesterol as well as lipid homeostasis. For research use only.
Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum. Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi: in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Binds cholesterol via its SSD domain.
| Product Name: | Human SCAP Recombinant Protein (N-His) |
| SKU: | GXRP11006 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | KIAA0199, SCAP, SREBP cleavage-activating protein, Sterol regulatory element-binding protein cleavage-activating protein |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser1063-Asp1279 |
| Calculated MW: | 25.40 kDa |
| UniProt: | Q12770 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
