The Human SCG3 (Secretogranin III) ELISA Kit is specifically designed for the accurate measurement of SCG3 levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.SCG3, also known as Secretogranin III, is a vital protein involved in the regulated secretion of peptide hormones and neurotransmitters. It plays a significant role in various physiological processes, making it a valuable biomarker for studying conditions such as neuroendocrine disorders, endocrine cancers, and neurodegenerative diseases. The Human SCG3 ELISA Kit provides researchers with a powerful tool for investigating the role of SCG3 in health and disease, paving the way for new insights and potential therapeutic interventions.
Product Name:
Human SCG3 (Secretogranin III) ELISA Kit
SKU:
HUES03485
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
