Human SECISBP2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser638-Leu854, with an N-terminal His tag. Predicted molecular weight: 26.71 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Selenocysteine insertion sequence-binding protein 2 (SECISBP2) is a ~95 kDa protein. mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid selenocysteine. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur. For research use only.
Product Name:
Human SECISBP2 Recombinant Protein (N-His)
SKU:
GXRP06387
Size:
50 µg, 100 µg, 1 mg
Species:
Human
Synonyms:
SECISBP2, SECIS-binding protein 2, SBP2, Selenocysteine insertion sequence-binding protein 2
Tag:
N-His
Expression Host:
E. coli
Protein Length:
Ser638-Leu854
Calculated MW:
26.71 kDa
UniProt:
Q96T21
Applications:
ELISA, Immunogen, SDS-PAGE, WB
Purity:
>90% as determined by SDS-PAGE.
Form:
Lyophilized
Formulation:
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution:
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
Storage:
Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Shipping:
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
