Human Secretoglobin Family 2A, Member 2 (SCGB2A2) ELISA Kit
The Human SCGB2A2 (Secretoglobin Family 2A Member 2) ELISA Kit is specially designed for the precise measurement of SCGB2A2 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for various research applications.SCGB2A2, also known as mammaglobin B, is a protein belonging to the secretoglobin family and is associated with breast and lung cancer. It serves as a potential biomarker for the early detection and monitoring of these cancers, providing valuable insights for diagnosis and treatment strategies.
By utilizing the Human SCGB2A2 ELISA Kit, researchers can gain valuable information about SCGB2A2 levels in human samples, contributing to a better understanding of cancer pathogenesis and potentially leading to the development of targeted therapies. This kit is a reliable and efficient tool for studying the role of SCGB2A2 in cancer progression and has the potential to advance research in this field.
Product Name:
Human Secretoglobin Family 2A, Member 2 (SCGB2A2) ELISA Kit
Product Code:
HUDL02614
Size:
96 Assays
Target:
SCGB2A2
Synonyms:
MGB1, MGBA, UGB2, Mammaglobin 1, Mammaglobin A, Uteroglobin 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
