Description
Human SETD2 Recombinant Protein (N-His)
Human SETD2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Leu1444-Val1834, with an N-terminal His tag. Predicted molecular weight: 47.38 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Histone-lysine N-methyltransferase SETD2 is a ~287 kDa protein. Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. For research use only.
It is capable of trimethylating unmethylated H3K36 (H3K36me0) in vitro. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction.
| Product Name: | Human SETD2 Recombinant Protein (N-His) |
| SKU: | GXRP11945 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EC:2.1.1.-, EC:2.1.1.359, HIF-1, HIF1, HIP-1, HYPB, Histone-lysine N-methyltransferase SETD2, Huntingtin yeast partner B, Huntingtin-interacting protein 1, Huntingtin-interacting protein B, KIAA1732, KMT3A, Lysine N-methyltransferase 3A, Protein-lysine N-methyltransferase SETD2, SET domain-containing protein 2, SET2, SETD2, hSET2, p231HBP |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Leu1444-Val1834 |
| Calculated MW: | 47.38 kDa |
| UniProt: | Q9BYW2 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
