Description
Human SHMT2 Recombinant Protein (N-His)
Human SHMT2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn30-His504, with an N-terminal His tag. Predicted molecular weight: 54.85 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Serine hydroxymethyltransferase, mitochondrial (SHMT2) is a ~55 kDa protein. Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis. For research use only.
Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs. Associates with mitochondrial DNA.
| Product Name: | Human SHMT2 Recombinant Protein (N-His) |
| SKU: | GXRP01460 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | SHMT2, Serine hydroxymethyltransferase, mitochondrial, SHMT, Glycine hydroxymethyltransferase, Serine methylase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn30-His504 |
| Calculated MW: | 54.85 kDa |
| UniProt: | P34897 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
