Human Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10) ELISA Kit
The Human Sialic Acid-Binding Ig-Like Lectin 10 (SIGLEC10) ELISA Kit is a powerful tool for quantifying SIGLEC10 levels in human samples like serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable asset for a variety of research purposes.SIGLEC10, also known as CD33L4, is a member of the SIGLEC family of proteins, which play roles in immune regulation and cell-cell interactions. SIGLEC10's involvement in immune responses and cell signaling makes it a key player in various disease processes, including inflammatory disorders and cancer.
As such, studying SIGLEC10 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.By utilizing the Human SIGLEC10 ELISA Kit, researchers can gain a deeper understanding of SIGLEC10's functions and its implications in health and disease. Whether investigating immune responses, cancer progression, or other biological processes, this kit offers the precision and reliability needed for impactful research outcomes.
Product Name:
Human Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
