Human Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) ELISA Kit
The Human Sialic Acid-binding Ig-like Lectin 8 (Siglec8) ELISA Kit is a reliable and sensitive tool for the accurate quantification of Siglec8 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring consistent and trustworthy results for a variety of research purposes.Siglec8 is a key protein involved in immune regulation and inflammatory responses, playing a critical role in various diseases including asthma, allergic diseases, and certain types of cancer.
By accurately measuring Siglec8 levels, researchers can gain valuable insights into disease pathogenesis and potential therapeutic interventions.Overall, the Human Siglec8 ELISA Kit is an essential resource for researchers studying immune-related disorders and seeking to uncover new therapeutic targets. Its high performance and reliability make it a valuable addition to any laboratory conducting research in the field of immunology and inflammation. Visit the website for more information and to order your kit today.
Product Name:
Human Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) ELISA Kit
Product Code:
HUDL02682
Size:
96 Assays
Target:
SIGLEC8
Synonyms:
SAF2, SIGLEC-8, SIGLEC8L, Sialoadhesin family member 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
