Human Signal Regulatory Protein Beta 1 (SIRPb1) ELISA Kit
The Human Signal Regulatory Protein Beta 1 (SIRPB1) ELISA Kit from Assay Genie is a powerful tool for the precise measurement of SIRPB1 levels in human samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Signal Regulatory Protein Beta 1 (SIRPB1) is a cell surface receptor that plays a crucial role in the regulation of immune responses and cell signaling pathways. By detecting and quantifying SIRPB1 levels, researchers can gain valuable insights into the mechanisms underlying immune system function, inflammation, and autoimmune disorders.
Whether investigating SIRPB1's involvement in cancer, autoimmune diseases, or inflammatory conditions, the Human SIRPB1 ELISA Kit provides researchers with a reliable and efficient solution for studying this important protein. Trust Assay Genie for high-quality ELISA kits that deliver consistent and dependable results for your research needs.
Product Name:
Human Signal Regulatory Protein Beta 1 (SIRPb1) ELISA Kit
Product Code:
HUDL02685
Size:
96 Assays
Target:
SIRPb1
Synonyms:
CD172-B, CD172b, SIRP-B1, SIRP-beta-1, CD172 antigen-like family member B
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
