The Human SIRT1 (Sirtuin 1) CLIA Kit is specially designed for the precise detection of Sirtuin 1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research studies.Sirtuin 1 is a key enzyme involved in regulating various cellular processes, including gene expression, metabolism, and aging.
It plays a critical role in maintaining cellular health and function, making it a valuable target for studying diseases such as cancer, diabetes, and neurodegenerative disorders. The Human SIRT1 CLIA Kit is an indispensable tool for researchers looking to explore the role of Sirtuin 1 in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human SIRT1 (Sirtuin 1) CLIA Kit
SKU:
HUES00876
Target:
Human SIRT1 (Sirtuin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SIRT1. You can calculate the concentration of Human SIRT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
93-107
89-102
Average (%)
107
99
96
1:4
Range (%)
98-112
91-106
94-107
Average (%)
105
99
100
1:8
Range (%)
90-105
100-115
84-99
Average (%)
97
108
91
1:16
Range (%)
84-96
100-113
88-101
Average (%)
91
105
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
102
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.94
567.22
1597.85
213.04
591.49
1582.25
Standard deviation
22.17
60.92
177.36
26.72
44.13
172.31
C V (%)
11.2
10.74
11.1
12.54
7.46
10.89
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SIRT1 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human SIRT1 in samples. No significant cross-reactivity or interference between Human SIRT1 and analogues was observed.
