The Human SIRT1 (Sirtuin 1) ELISA Kit is specifically designed for the precise measurement of Sirtuin 1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Sirtuin 1 is a key protein involved in regulating cellular processes such as metabolism, aging, and stress response. It plays a crucial role in various physiological and pathological conditions, including metabolic disorders, neurodegenerative diseases, and cancer. The ability to accurately measure Sirtuin 1 levels can provide valuable insights into these conditions and aid in the development of potential treatment strategies.Overall, the Human SIRT1 ELISA Kit from Assay Genie is a reliable tool for researchers looking to study Sirtuin 1 and its impact on human health.
Product Name:
Human SIRT1 (Sirtuin 1) ELISA Kit
SKU:
HUES02560
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
