Description
Human SMAD3 Recombinant Protein (N-His)
Human SMAD3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp220-Ser425, with an N-terminal His tag. Predicted molecular weight: 25.5 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. SMAD family member 3 (SMAD3) is a ~48 kDa protein. Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. For research use only.
Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.
| Product Name: | Human SMAD3 Recombinant Protein (N-His) |
| SKU: | GXRP01970 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Mad3, JV15-2, Mothers against decapentaplegic homolog 3, SMAD family member 3, Smad3, SMAD3, MADH3, hMAD-3, SMAD 3, MAD homolog 3, hSMAD3, Mothers against DPP homolog 3 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asp220-Ser425 |
| Calculated MW: | 25.5 kDa |
| UniProt: | P84022 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
