The Human Sorting Nexin 13 (SNX13) ELISA Kit is a powerful tool for the accurate measurement of SNX13 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.SNX13 is a key protein involved in intracellular trafficking and endosomal sorting, playing a crucial role in processes such as receptor recycling and signal transduction. Dysregulation of SNX13 has been implicated in various diseases including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With the Human SNX13 ELISA Kit, researchers can accurately quantify SNX13 levels in human samples to further understand its role in cellular processes and disease pathology, ultimately advancing our knowledge and potentially leading to new treatment strategies.
Product Name:
Human Sorting Nexin 13 (SNX13) ELISA Kit
Product Code:
HUDL02733
Size:
96 Assays
Target:
SNX13
Synonyms:
RGS-PX1, RGS domain- and PHOX domain-containing protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
