Human TFE3 Recombinant Protein (N-His) (GXRP04581)

Human TFE3 Recombinant Protein (N-His)

Human TFE3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn185-Ser281, with an N-terminal His tag. Predicted molecular weight: 12.58 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Transcription factor E3 (TFE3) is a ~61 kDa protein. Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. For research use only.

Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes.